Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Run.fm
Step 3 – Configuring the Plate Document with Tasks
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 3-17
3. Repeat steps 1 through 2 to apply any remaining tasks to the plate document.
4. Choose from the following options:
Assigning
Standards for
Absolute
Quantification
For the SDS software to create a standard curve for quantification of unknown
samples, you must apply the Standard task and quantity values to your absolute
quantification plate document.
1. In the plate grid of the SDS software, select the replicate wells containing the
first standard in the dilution series.
2. In the well inspector, click the field in the Task column for the appropriate
detector entry, and select the Standard from the drop-down list.
3. In the well inspector, click the field in the Quantity column for the appropriate
detector, enter a quantity or concentration for the standard in the appropriate
unit of measurement (starting copy number, picograms, nanograms, etc.), and
press Enter.
The software labels the selected standard wells with the specified quantity.
4. Repeat steps 1 through 3 to configure the plate with the other sets of replicate
standard wells on the plate.
When finished, the plate grid should contain a complete set of replicate wells
labeled with the Standard task and assigned quantities that the software will
use to compute the standard curve for the run.
5. Do one of the following:
• If necessary, set the passive reference for the plate document as explained on
“Step 4 – Setting the Passive Reference and Omitting Wells” on page 3-18.
• Otherwise, program the method for your run as explained in
“Step 5 – Programming the Plate Document Method” on page 3-19.
If constructing a
plate document for…
Then…
absolute quantification assign quantities to the standard wells of the plate
document as explained below.
• allelic discrimination
• relative quantification
• dissociation curve analysis
• If necessary, set the passive reference for the
plate document as explained on page 3-18.
• Otherwise, program the method for your run as
explained on page 3-19.
Quantity field
Selected wells
(quantity applied
to selection)

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English