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Applied Biosystems 7900HT Getting Started Guide
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DRAF
T
June 23,
2003 9:39 a
m, MFC_QSG_T
itle.fm
Applied Biosystems 7900HT
Micr
o Fluidic Car
d
Getting Started Guide
FINAL DRAF
T June 5, 2003
Please r
eturn to Clarissa Kao (x6673) MS 458 by June 11.
2
Table of Contents
Default Chapter
3
Table of Contents
3
Safety
5
Safety Alert Words
5
Chemical Hazard Warning
5
Chemical Safety Guidelines
5
About Msdss
6
Obtaining Msdss
6
Site Preparation and Safety Guide
7
Waste Disposal
7
How to Obtain Services and Support
7
Introduction
8
About the Getting Started Guide
8
About the Micro Fluidic Card
8
About Micro Fluidic Card Chemistry
9
Preventing Contamination
9
Process Flowchart
10
Materials and Equipment
11
Components and Storage Conditions
11
Ordering Additional Cards or Reagents
12
Items Required but Not Supplied
13
Notes about Micro Fluidic Card Experiments
16
About the Comparative C
16
Significance of the Calibrator Sample
16
T Method of Relative Quantification
16
Design Guidelines
16
Performing Reverse Transcription
17
Summary
17
Where You Are in the Procedure
17
Guidelines for Preparing RNA
18
Starting Amount of Total RNA
18
Storing Cdna
18
Preparing and Running the Micro Fluidic Cards (PCR)
19
Summary
19
Where You Are in the Procedure
19
Process Overview
20
Guidelines for Preparing the PCR MIX
21
Amount of Cdna in the PCR MIX
21
Preparing the Sample-Specific PCR MIX
21
Guidelines for Loading Cards and Operating the Centrifuge
23
Creating Micro Fluidic Card Documents
24
Performing the Micro Fluidic Card Run
30
Performing Real-Time Data Analysis
33
Summary
33
Where You Are in the Procedure
33
Absolute Vs. Relative Quantification
34
Additional Tasks Related to Micro Fluidic Card Use
35
References
36
Appendix A Micro Fluidic Card Information CD
37
About the Micro Fluidic Card Information CD
37
Assay Information File
37
Card Map Files
40
SDS Setup File
41
Index
43
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Applied Biosystems 7900HT Specifications
General
Brand
Applied Biosystems
Model
7900HT
Category
Laboratory Equipment
Language
English
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