Applied Biosystems 7900HT Getting Started Guide

DRAFT
June 23, 2003 9:39 am, MFC_QSG_text.fm
16 Applied Biosystems Micro Fluidic Card Getting Started Guide
Process
Overview
The PCR process consists of the following procedures:
1. Preparing the sample-specific PCR mix.
See page 17.
2. Loading the Micro Fluidic Cards.
See Chapter 4 of the user guide for the 7900HT system.
3. Centrifuging the Micro Fluidic Cards.
See Chapter 4 of the user guide for the 7900HT system and
page 19 for Micro Fluidic Card-specific guidelines on
centrifuge operation.
4. Sealing the Micro Fluidic Cards and trimming-off fill
reservoirs.
See Chapter 4 of the user guide for the 7900HT system.
IMPORTANT! The sealing process is unidirectional. Once
you go over the card in one direction, you cannot reverse the
process.
Note: Sealing tape is not required for the card sealer. The
sealer makes grooves into the foil side of the card, sectioning
off the sample pocket from the main channel.
5. Creating Micro Fluidic Card Documents.
See page 20.
6. Performing Micro Fluidic Card Runs.
See page 26.

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English