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Applied Biosystems 7900HT Getting Started Guide

Applied Biosystems 7900HT
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DRAFT
June 23, 2003 9:39 am, MFC_QSG_TOC.fm
Contents
Applied Biosystems Micro Fluidic Card Getting Started Guide iii
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Safety Alert Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Chemical Hazard Warning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Chemical Safety Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
About MSDSs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Obtaining MSDSs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Site Preparation and Safety Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Waste Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
How to Obtain Services and Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
About the Getting Started Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
About the Micro Fluidic Card . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
About Micro Fluidic Card Chemistry . . . . . . . . . . . . . . . . . . . . . . . . . 5
Preventing Contamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Process Flowchart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Materials and Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Components and Storage Conditions . . . . . . . . . . . . . . . . . . . . . . . . 7
Ordering Additional Cards or Reagents . . . . . . . . . . . . . . . . . . . . . . . 8
Items Required But Not Supplied . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Notes About Micro Fluidic Card Experiments . . . . . . . . . . . . . . . . . . . . . 12
About the Comparative C
T
Method of Relative Quantification . . . . . 12
Significance of the Calibrator Sample . . . . . . . . . . . . . . . . . . . . . . . 12
Design Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Performing Reverse Transcription . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Where You Are in the Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Guidelines for Preparing RNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Starting Amount of Total RNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Storing cDNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Preparing and Running the Micro Fluidic Cards (PCR) . . . . . . . . . . . . . . 15
Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Where You Are in the Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Process Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

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Applied Biosystems 7900HT Specifications

General IconGeneral
BrandApplied Biosystems
Model7900HT
CategoryLaboratory Equipment
LanguageEnglish

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