Applied Biosystems 7900HT Getting Started Guide

DRAFT
June 23, 2003 9:39 am, MFC_QSG_TOC.fm
iv Applied Biosystems Micro Fluidic Card Getting Started Guide
Guidelines for Preparing the PCR Mix . . . . . . . . . . . . . . . . . . . . . . . 17
Amount of cDNA in the PCR Mix . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Preparing the Sample-Specific PCR Mix . . . . . . . . . . . . . . . . . . . . . 17
Guidelines for Loading Cards and Operating the Centrifuge . . . . . 19
Creating Micro Fluidic Card Documents . . . . . . . . . . . . . . . . . . . . . 20
Performing the Micro Fluidic Card Run . . . . . . . . . . . . . . . . . . . . . . 26
Performing Real-Time Data Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Where You Are in the Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Absolute vs. Relative Quantification . . . . . . . . . . . . . . . . . . . . . . . . 30
Additional Tasks Related to Micro Fluidic Card Use . . . . . . . . . . . . . . . 31
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Appendix A Micro Fluidic Card Information CD . . . . . . . . . . . . . . . . . 33
About the Micro Fluidic Card Information CD . . . . . . . . . . . . . . . . . 33
Assay Information File . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Card Map Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
SDS Setup File . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English