Applied Biosystems 7900HT Getting Started Guide

DRAFT
June 23, 2003 9:39 am, MFC_QSG_text.fm
Performing Real-Time Data Analysis
Applied Biosystems Micro Fluidic Card Getting Started Guide 29
Performing Real-Time Data Analysis
Summary
Real-time is the term used to describe the category of sequence
detection runs in which the ABI P
RISM
®
7900HT Sequence
Detection System is used to measure the fluorescence of a biological
sample during thermal cycling. Real-time experiments can be used to
achieve both qualitative and quantitative measurements.
Where You Are in
the Procedure
You
Are Here
Performing Real-Time Data Analysis
(7900HT system and SDS software)
1. Analyze the PCR sequence
detection run data.
2. Select analysis settings.
3. Eliminate outlying
amplification.
4. Export the analyzed data
for relative quantification.
Performing Real-Time Data
Analysis
(7900HT system and SDS
software)
Performing Reverse Transcription
(cDNA generation)
Interpreting Results
Loading, Centrifuging, Sealing and
Running the Micro Fluidic Cards

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English