Applied Biosystems 7900HT Getting Started Guide

DRAFT
June 23, 2003 9:39 am, MFC_QSG_text.fm
Performing Reverse Transcription
Applied Biosystems Micro Fluidic Card Getting Started Guide 13
Performing Reverse Transcription
Summary
Synthesis of cDNA from total RNA samples using the High Capacity
cDNA Archive Kit (PN 4322171) is the first step in the two-step
RT-PCR gene expression quantification experiment.
Use the manual method for converting total RNA into cDNA, as
specified in the High Capacity cDNA Archive Kit Protocol
(PN 4322169).
Note: The protocol is not shipped with the High Capacity cDNA
Archive Kit. You must download the protocol from the
Applied Biosystems Documents on Demand Web site at
http://docs.appliedbiosystems.com/search.taf
To search for the document, select ABI P
RISM
™
6100 Nucleic Acid
PrepStation under Products and click Search. The protocol is listed
under the Protocols heading.
Where You Are in
the Procedure
You
Are Here
1. Prepare total RNA
samples.
2. Use the manual method of
the High Capacity cDNA
Archive Kit to generate
cDNA.
Performing Reverse
Trans cr ipt io n
(cDNA generation)
Performing Reverse Transcription
(cDNA generation)
Performing Real-Time Data Analysis
(7900HT system and SDS software)
Preparing and Running the Micro
Fluidic Cards (PCR)
Interpreting Results

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English