Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Basic.fm
Section 4.2 Running an Individual Plate
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 4-23
Section 4.2 Running an Individual Plate
In This Section
Saving the Plate Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-24
Running a Single Plate (Using the SDS Software) . . . . . . . . . . . . . . . . . . . . . . . . 4-25
After the Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-29
Pre-Run
Checklist
The following tasks must be complete to run plates on the 7900HT instrument. See
the associated page number for details on each procedure.
Workflow
Overview
Done Check
See Page
A background run has been performed in the last week 7-16
A pure dye run has been performed in the last 6 months 7-20
The instrument tray does not contain a plate
IMPORTANT! The instrument tray must be empty to begin a run. If
the instrument tray contains a plate, you must eject and remove it.
4-44
1. Save the SDS Plate Document (see page 4-24).
2. Load and run the prepared optical plate or Low Density Array (see page 4-25).
3. Analyze the run data.
– Allelic Discrimination Analysis (see page 5-5).
– Absolute Quantification Analysis (see page 6-5).
– Relative Quantification Analysis (see page 6-15).
– Dissociation Curve Analysis (see page 6-37).

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English