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Applied Biosystems 7900HT - Section 5.1 Allelic Discrimination

Applied Biosystems 7900HT
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DRAFT
September 1, 2004 11:39 am, CH_End-Point.fm
Section 5.1 Allelic Discrimination
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 5-5
Section 5.1 Allelic Discrimination
In This Section
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
Before You Begin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-9
Analysis Checklist. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
Opening the Run Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-11
Analyzing an Allelic Discrimination Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-12
Calling and Scrutinizing Allelic Discrimination Data . . . . . . . . . . . . . . . . . . . . . 5-13
After the Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-18

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