Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:38 am, CH_Overview.fm
Managing Sequence Detection System Data
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 1-17
Managing Sequence Detection System Data
System
Operation and
Dataflow
Data management strategy is a crucial element of successfully integrating the
Applied Biosystems 7900HT Fast Real-Time PCR System into a laboratory
workflow. During a single 24-hour period of real-time operation, the 7900HT
instrument can produce more than 200 MB of data. To successfully manage the
information produced by the 7900HT instrument, you should have a basic
understanding of how data is collected, stored, and processed throughout the
operation of the instrument.
Standard Modes
of Operation
The Applied Biosystems 7900HT Fast Real-Time PCR System has three standard
modes of operation depending on the accessories purchased with the base instrument
• Stand-Alone Operation (see below)
• Automated Operation (see page 1-18)
• Database Operation (see page 1-18)
Stand-Alone Operation
This mode uses the most basic configuration of the Applied Biosystems 7900HT Fast
Real-Time PCR System (Figure 1-11) where the 7900HT instrument is used without any
additional components. In this configuration, a technician runs plates individually using
the SDS software which stores all data on the computer hard drives.
Figure 1-11 Stand-Alone Operation of the 7900HT Instrument
Hard drive(s)
Computer
Serial
Cable
Plate Document
Created
*.sds
file
SDS Software
Thermal Cycling and
Sequence Detection
Instrument Firmware
*.sdt
file
Data Collection
Plate Document Used
to Run a Plate
Plate Document
Opened and Analyzed
GR2179
7900HT raven
instrument front
7900HT FAST Real-Time PCR System
1
2
3
4
5
6
Raw Data Saved to
the Plate Document
Applied Biosystems 7900HT
Fast Real-Time PCR System

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English