DRAFT
September 1, 2004 11:38 am, CH_Overview.fm
Chapter 1 Product Overview
1-18 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Automated Operation
In this mode, the Applied Biosystems 7900HT Fast Real-Time PCR System uses an
Automation Accessory (Zymark Twister Microplate Handler and fixed-position bar
code reader), to provide high-throughput operation suitable for small- to
medium-scale studies. With this configuration (Figure 1-12), a technician can run
batches of plates unattended using the Automation Controller Software. As in
Stand-Alone operation, the computer stores all data for operation of the instrument
and provides the software for analyzing the run data.
Figure 1-12 Automated Operation of the of the 7900HT Instrument
Database Operation
For this mode, the Applied Biosystems 7900HT Fast Real-Time PCR System uses
the SDS Enterprise Database and the Automation Accessory, to provide maximum
high-throughput potential. In this configuration (Figure 1-13 on page 1-19), the
7900HT instrument has the storage capacity and analysis capability to support
medium- to large-scale gene expression and genotyping studies. Moreover, the SDS
Enterprise Database functions as a repository for all SDS-related data produced by
the networked client computers used for data collection and analysis.
Hard drive(s)
Computer
Serial
Cable
Automation Controller
Software
Thermal Cycling and
Sequence Detection
Instrument Firmware
GR2009
7900HT
Front view with Robot
7900HT FAST Real-Time PCR System
GR2009
Data Collection
2
3
4
5
Raw Data Saved to
the Plate Documents
Plate document files
added to and run from
the plate queue
Plate Documents
Created
1
SDS Software
Plate Document
Opened and Analyzed
6
*.sds
files
*.sdt
file
Applied Biosystems 7900HT
Fast Real-Time PCR System