Applied Biosystems 7900HT - Chapter 3 Preparing a Run

DRAFT
September 1, 2004 11:39 am, CH_Run.fm
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 3-1
3
Preparing a Run 3
In This Chapter
Notes for Database Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Before You Begin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Workflow Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Quick Review: Powering On the 7900HT Instrument. . . . . . . . . . . . . . . . . . . . . . . 3-8
Step 1 – Creating a Plate Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
Step 2 – Applying Detectors and Markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
Step 3 – Configuring the Plate Document with Tasks . . . . . . . . . . . . . . . . . . . . . 3-16
Step 4 – Setting the Passive Reference and Omitting Wells . . . . . . . . . . . . . . . . . 3-18
Step 5 – Programming the Plate Document Method. . . . . . . . . . . . . . . . . . . . . . . 3-19
Step 6 – Saving the Plate Document as a Template. . . . . . . . . . . . . . . . . . . . . . . . 3-24
Step 7 – Creating a Plate Document from the Template . . . . . . . . . . . . . . . . . . . . 3-26
Step 8 – Applying Sample and Plate Information. . . . . . . . . . . . . . . . . . . . . . . . . 3-28
Step 9 – Running the Plate on the 7900HT Instrument. . . . . . . . . . . . . . . . . . . . . 3-29