DRAFT
September 1, 2004 11:39 am, CH_Basic.fm
Chapter 4 Operating the Instrument
4-12 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
About the Centrifuge System
After the fill reservoirs of a Low Density Array have been loaded with the cDNA
sample or control, the centrifuge system is required to distribute the cDNA sample or
control to the reaction wells. The centrifuge system supports the simultaneous
loading of up to 12 Low Density Arrays.
Required
Centrifuge and
Accessories
To properly centrifuge the Low Density Arrays, you will need the following
centrifuge and centrifuge accessories:
•Sorvall
®
Legend T Centrifuge
Figure 4-7 Sorvall Legend T Centrifuge
•Sorvall
®
/Heraeus “Tool-less” 750-mL Swing-Out Rotor Body
•A set of Sorvall
®
/Heraeus Custom Buckets (four), each with its own card holder
(Contact your Applied Biosystems representative to order the custom buckets.)
• Current firmware
If you are upgrading an existing Sorvall Legend T or Legend RT Centrifuge,
you may also need to upgrade the firmware so that it can recognize the Low
Density Array bucket type.
Centrifuge
System
Components
Figure 4-8 illustrates the centrifuge system components.
Figure 4-8 Centrifuge System Components
GR2170
GR2152
GR2153
GR2154
Centrifuge rotor
Provides the rotational drive for
a set of four centrifuge buckets
Card holder
Supports the Low Density
Array’s fill reservoirs during
centrifugation
Bucket
Contains the Low Density
Arrays and card holder
during centrifugation