Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Trouble.fm
Chapter 8 Troubleshooting
8-2 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Troubleshooting Table
Overview
The following table is designed to help you troubleshoot most of the problems you
may encounter while using the Applied Biosystems 7900HT Fast Real-Time PCR
System.
The information in the table is arranged by category as follows:
• Chemistry problems
• Run problems
• Instrument and Automation Accessory Problems
Each category contains subcategories, followed by a brief description of the
symptoms you might encounter.
To use this table, look for the category and the symptom you are experiencing. The
page number in the right-hand column corresponds to a description of the possible
cause(s) and recommended action(s) for that particular problem.
Table 8-1 Troubleshooting Table
Category Symptom Page
Chemistry and Run Problems
Chemistry Low Precision 8-5
Irreproducibility
Software Installation
SDS Software Unable to finish installation
(Install program appears to b e frozen)
8-5
Installation Interrupted
Run Problems
Background Runs Software will not extract background data 8-9
Background is too high (greater than 2500)
Pure Dye Runs Software will not extract pure dye data 8-11
Raw data from pure dye run appears strange
Signals plateau (saturation)
Signal is too low (< 10,000 FSU)
More than two outliers per dye in a single row
Real-Time Runs (Quantitative PCR and Dissociation Curves) 8-12
End-Point Runs (Allelic Discrimination) 8-13

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English