DRAFT
September 25, 2007 1:07 am, 4376782_Maintain_the_System.fm
Chapter 6 Maintain the System
Regular Maintenance
107
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
Notes
2. For each dye present on the plate, confirm the grouping of the dye spectra:
a. In the plate layout, press Ctrl, then select the wells that contain the same dye.
b. Inspect the raw data. For each spectrum, confirm that the peak is:
• Within the detectable range for the instrument.
• Free of irregular spectral peaks.
• Present in the correct channel for the dye (see “StepOne
™
System Dye
Spectra” on page 108 or “StepOnePlus
™
System Dye Spectra” on page 109).
If a spectrum does not comply with the criteria above, troubleshoot the problem as
described in
“Dye Calibration Failure” on page 134
.
Note: Among wells containing the same dye, variations in spectral position and
peak position are caused by minor differences in the optical and excitation energy
between the individual wells.
3. If all spectra are acceptable, click Plate 2 from the View Details menu.
4. Inspect the raw data for the as described in step 2b.
5. If all spectra are acceptable, click Finish.
6. When prompted to save the dye calibration, click Ye s .
1
2b/4
5
6
3
2a
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