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Applied Biosystems 2720 User Manual

Applied Biosystems 2720
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B-2 Supplied Methods
About the Methods
Five Precoded
Methods
The Applied Biosystems 2720 Thermal Cycler supplies you with five precoded
methods stored under the user name <<ab>>:
AmpliTaq Gold
®
BigDye
®
Terminator
General PCR
Time Release PCR
Touchdown PCR
AmpliTaq Gold
The AmpliTaq Gold protocol specifies a 5-minute pre-PCR heat step, required for the
activation of AmpliTaq Gold
®
DNA Polymerase. This additional step provides
seamless “hot start” PCR and replaces labor intensive methods such as manual hot
start or wax bead-mediated hot start techniques.
Utilizing hot start techniques helps to minimize the formation of primer-dimers or
non-specific products, thus increasing specificity and sensitivity of PCR.
You can find further information on AmpliTaq Gold DNA Polymerase in the product
insert (P/N N808-0241) or at the Applied Biosystems Web site.
95.0
5:00
95.0
0:15
55.0
0:15
72.0
0:30
72.0
7:00
2 Holds3 Tmp 35 Cycles1 Hld
4.0
F1 F3 F4 F5F2
Start
Return
Method: AmpliTaq Gold
®

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Applied Biosystems 2720 Specifications

General IconGeneral
BrandApplied Biosystems
Model2720
CategoryLaboratory Equipment
LanguageEnglish

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