Applied Biosystems 2720 - About the Methods

B-2 Supplied Methods
About the Methods
Five Precoded
Methods
The Applied Biosystems 2720 Thermal Cycler supplies you with five precoded
methods stored under the user name <<ab>>:
♦ AmpliTaq Gold
®
♦ BigDye
®
Terminator
♦ General PCR
♦ Time Release PCR
♦ Touchdown PCR
AmpliTaq Gold
The AmpliTaq Gold protocol specifies a 5-minute pre-PCR heat step, required for the
activation of AmpliTaq Gold
®
DNA Polymerase. This additional step provides
seamless “hot start” PCR and replaces labor intensive methods such as manual hot
start or wax bead-mediated hot start techniques.
Utilizing hot start techniques helps to minimize the formation of primer-dimers or
non-specific products, thus increasing specificity and sensitivity of PCR.
You can find further information on AmpliTaq Gold DNA Polymerase in the product
insert (P/N N808-0241) or at the Applied Biosystems Web site.
95.0
5:00
95.0
0:15
55.0
0:15
72.0
0:30
72.0
7:00
2 Holds3 Tmp 35 Cycles1 Hld
4.0
F1 F3 F4 F5F2
Start
Return
Method: AmpliTaq Gold
®