Runs 3-5
Loading Samples
Procedures for
Loading Samples
The following procedures describe how to load samples for:
♦ Tubes with attached caps.
♦ 96-well reaction plate.
♦ 96-well tray/retainer assembly.
Note Do not use mineral oil or glycerine in the sample block or as a vapor barrier over the
PCR reaction mixture in the tubes. The MicroAmp Reaction Tubes fit tightly in the wells and a
heated cover exerts an even pressure on all tubes and eliminates condensation on the tubes.
Loading Tubes with
Attached Caps
Loading the 96-Well
Reaction Plate
Loading the 96-Well
Tray/Retainer
To load tubes with attached caps:
Step Action
1 Set the 96-well tray on a splash-free support base.
2 Place the reaction tubes in the tray.
3 Pipette the samples into the reaction tubes.
4 Cap the tubes.
See “Placing the Sample Tray or Plate onto the Sample Block” on page 3-6.
To load the 96-well reaction plate:
Step Action
1 Place the reaction plate on the splash-free support base.
2 Pipette the samples into the sample wells.
3 Cap the tubes using either the MicroAmp 96-Well Full Plate Cover or the MicroAmp
Caps, 8 Caps/Strip.
See “Placing the Sample Tray or Plate onto the Sample Block” on page 3-6.
To load the 96-well tray/retainer:
Step Action
1 Place the tray on the splash-free support base.
2 Load tubes onto the tray, either using single tubes or using the 8-strip tubes.
3 Place retainer over the tubes.
4 Pipette the sample into the tubes.
5 Cap the tubes using either the MicroAmp 96-Well Full Plate Cover or the
MicroAmp Caps, 8 Caps/Strip.
See “Placing the Sample Tray or Plate onto the Sample Block” on page 3-6.
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