Chapter 5 Performing the Background Calibration and Optical Calibration
Analyzing the Background Calibration Data
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 57
Notes
5
4. In the plate document, select the Results tab,
then select the Spectra tab.
5. Select all wells of the plate document.
6. Inspect the raw data for irregular spectral peaks
that exceed the following fluorescent standard
units (FSU):
If one or more wells produce raw spectra that
exceed the specified FSU, the background plate
or the sample block could contain a fluorescent
contaminant. Determine the source of the
contamination. See “Troubleshooting” on
page 64.
7. Select FileClose.
a
b
Click to select
all wells
Possible
contamination
Filter FSU
A, B, C, D (7300/7500) >72,000
E (7500) >90,000
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