Applied Biosystems 7500 Fast User Manual

Appendix C
Adding the Custom Dye to the Software
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 131
Notes
C
Adding the Custom Dye to the Software
1. In the SDS software, select Tool sDye
Manager.
2. In the Dye Manager dialog box, click .
3. In the Add Dye dialog box, enter a name for the
custom dye, then click .
4. Click .
Run the custom pure dye plate (see Chapter 6,
“Performing the Pure Dye Calibration.”)
Select
Click
Enter a name for
the dye
Click

Applied Biosystems 7500 Fast Specifications

General

Instrument Type	Real-Time PCR System
Temperature Uniformity	±0.5 °C
Heating Rate	4.4 °C/sec
Ramp Rate (heating)	4.4 °C/sec
Detection Channels	5
Data Acquisition	Continuous
Thermal Cycler Block	96-well
Sample Capacity	96-well
Format	96-well plate
Reaction Volume	10–100 μL
Temperature Range	4°C to 100°C
Temperature Accuracy	±0.25 °C (35–100 °C)
Detection Method	Fluorescence
Excitation Source	LED
Excitation Wavelengths	470 nm, 520 nm, 580 nm, 623 nm
Dyes	SYBR Green, FAM, VIC, NED, ROX, Texas Red
Power Requirements	100–240 VAC, 50/60 Hz