Applied Biosystems 7500 Fast User Manual

Chapter 5 Performing the Background Calibration and Optical Calibration
Analyzing the Background Calibration Data
58 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Unloading the
Plate
PHYSICAL INJURY HAZARD. During instrument operation, the
sample block can be heated to 100 °C. Before performing the following procedure, be
sure to wait until the sample block reaches room temperature.
1. When the run is complete, remove the background plate:
a. Press the tray to open it.
b. Remove the background plate.
c. Press the tray to move it into the instrument.
2. Place the background
plate inside its packaging
sleeve, then return it to the
spectral calibration kit in
the freezer.
IMPORTANT! Do not discard the background plate. If you store the background
plate in its original packaging sleeve, you can use the plate up to three times after
you open it.
Continue with:
• 7300 system – “Performing the Pure Dye Calibration” on page 67.
• 7500/7500 Fast system – “Performing the Optical Calibration for the
7500/7500 Fast System” on page 59.
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Applied Biosystems 7500 Fast Specifications

General

Instrument Type	Real-Time PCR System
Temperature Uniformity	±0.5 °C
Heating Rate	4.4 °C/sec
Ramp Rate (heating)	4.4 °C/sec
Detection Channels	5
Data Acquisition	Continuous
Thermal Cycler Block	96-well
Sample Capacity	96-well
Format	96-well plate
Reaction Volume	10–100 μL
Temperature Range	4°C to 100°C
Temperature Accuracy	±0.25 °C (35–100 °C)
Detection Method	Fluorescence
Excitation Source	LED
Excitation Wavelengths	470 nm, 520 nm, 580 nm, 623 nm
Dyes	SYBR Green, FAM, VIC, NED, ROX, Texas Red
Power Requirements	100–240 VAC, 50/60 Hz