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Applied Biosystems ViiA 7 Real User Manual

Applied Biosystems ViiA 7 Real
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6
Perform an experiment
Applied Biosystems ViiA
7 Real-Time PCR System Quick Reference Guide
Design the experiment
1
Define experiment name
and type (Experiment
Properties screen)
Define the following properties:
Experiment name
User name of the experiment owner
(Optional) Barcode for the plate or array card
(Optional) Comments
•Block type: 384-well, Array Card, 96-well (0.2mL), or Fast 96-well (0.1mL)
Experiment type: Standard Curve, Relative Standard Curve, Comparative C
T
(∆∆C
T
), Melt
Curve, Genotyping, or Presence/Absence
2
Select the reagents
(Experiment Properties
screen)
Select reagents to use in the experiment:
Note: If you select SYBR
®
Green as the reagent, then you have the option of including a melt curve
for that experiment.
3
Define the instrument
run properties
(Experiment Properties
screen)
Ramp speed: Standard or Fast
(Genotyping and Presence/Absence) Collect data during Pre-PCR Read, Amplification, or Post-
PCR Read
(Experiments using SYBR
®
Green) Select whether or not to include a melt curve.
(Melt Curve) Select whether or not to include PCR.
4
Define targets, samples,
biological replicates,
and dye (Define screen)
(All experiments except Genotyping) Define Targets.
(Genotyping) Define SNPs.
(All experiments) Define Samples.
(Optional) (Standard Curve, Relative Standard Curve, and Comparative C
T
) Define Biological
Replicate Groups.
(All experiments except Presence/Absence) Select the Passive Reference dye.
(Relative Standard Curve and Comparative C
T
) Select the Reference Sample and the Endogenous
Control.
5
Assign targets and
samples (Assign screen)
(All experiments except Genotyping) Assign targets, tasks, and samples to wells. Tasks include
Unknown, Standard, Positive, and Negative controls, depending on experiment type.
(Genotyping) Assign SNP assays, tasks, and samples to wells.
(Standard Curve and Relative Standard Curve) Define and set up standards: Click Define and Set
Up Standards, select a target, define the standard curve, then select and arrange wells for the
standards.
(Optional) (Standard Curve, Relative Standard Curve, and Comparative C
T
) Assign Biological
Replicate Groups to wells.
Experiment type Reagent options
Standard Curve TaqMan
®
Reagents, SYBR
®
Green Reagents, or Other
Relative Standard Curve TaqMan
®
Reagents, SYBR
®
Green Reagents, or Other
Comparative C
T
TaqMan
®
Reagents, SYBR
®
Green Reagents, or Other
Melt Curve SYBR
®
Green Reagents or Other
Genotyping TaqMan
®
Reagents or Other
Presence/Absence TaqMan
®
Reagents or Other

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Applied Biosystems ViiA 7 Real Specifications

General IconGeneral
BrandApplied Biosystems
ModelViiA 7 Real
CategoryLaboratory Equipment
LanguageEnglish

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