Applied Biosystems ViiA 7 Real User Manual

18
Maintain the ViiA
™
7 System
Applied Biosystems ViiA
™
7 Real-Time PCR System Quick Reference Guide
4
Verify success Perform a background calibration.
If the contamination remains, remove the sample block (step 1) and clean the contaminated wells
(step 2), then clean the contaminated wells again using 95% ethanol solution:
a. Pipette a small volume of 95% ethanol solution into each contaminated well.
b. In each contaminated well, pipette the solution up and down several times to rinse the well.
c. Pipette the ethanol solution to a waste beaker.
IMPORTANT! Always use deionized water to rinse wells after cleaning with bleach or ethanol
solution.
d. Clean the wells again (step 2), to rinse the wells of the sample block.
e. Perform a background calibration, to verify that you have eliminated the contamination.
If the contamination remains, remove the sample block and clean the contaminated wells (step 2),
then clean the contaminated wells again using 10% bleach solution:
a. Pipette a small volume of 10% bleach solution into each contaminated well.
b. In each contaminated well, pipette the solution up and down several times to rinse the well.
c. Pipette the bleach solution to a waste beaker.
IMPORTANT! Always use deionized water to rinse wells after cleaning with bleach or ethanol
solution.
d. Clean the wells again (step 2) to rinse the wells of the sample block.
e. Perform a background calibration, to verify that you have eliminated the contamination.
If the contamination remains, contact Applied Biosystems Technical Support.

Applied Biosystems ViiA 7 Real Specifications

General

Brand	Applied Biosystems
Model	ViiA 7 Real
Category	Laboratory Equipment
Language	English