BBE BenthoTorch - Reference Method for Chlorophyll-a

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Operating the bbe BenthoTorch

bbe BenthoTorch Manual Page 20

green:

0.7µg/cm’2

more <- -> ESC

cyanos:

0.7µg/cm’2

more <- -> ESC

diatoms:

0.7µg/cm’2

more <- -> ESC

• More switches between the following items:

• Automatic alternating of the tree algae classes

• Display of the concentration of cyanobacteria

• Display of the concentration of green algae

• Display of the concentration of diatom

• Display of the time / date of the measurement.

• Use the arrow keys to display data of another point in time.

• Press “ESC” to return to the start measurement item.

NTERVAL

CONTINUOUS MEASUREMENT

• Start the BenthoTorch

• Use a carrying cable/rope to secure the instrument

• Ensure that the connector is closed with the cap / dummy connector

• Do not exceed the maximum depth (10 m / 100 m).

• Use the continuous mode (settings -> mode -> continuous)

• Adjust measuring time and interval

• Go to “Start -> Start continuous measurement”

• Apply the BenthoTorch in to the benthic algae. Ensure that the red foam shadows the algae from the light.

• To stop the BenthoTorch, turn it upside down and back between two measurements when the BenthoTorch

switches off because of the selected measuring interval. If the measuring interval is 0 s, use the exit key

between two measurements:

• To read the data, go to “Data -> view”

Reference method for chlorophyll-a measurement for validation of the bbe BenthoTorch

UBSTRATE USED FOR REFERENCE

For test purposes, substrata used are exposed, flat materials which are non-fluorescent in visible light and easy

to scrape:

a. Black PVC plates (4x6cm)

b. Gray PVC plates (4,5x7,5cm)

c. Glass microscopic slides darkened on one side to omit the “double sidedness problem”.