Beckman Coulter Avanti J-E User Manual

PN JR-IM-10AG
October 2014
Instructions For Use
Rotors and Tubes
For Beckman Coulter
J2, J6, and Avanti J Series Centrifuges
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

Beckman Coulter Avanti J-E Specifications

General

Model	Avanti J-E
Manufacturer	Beckman Coulter
Maximum Speed	21, 000 rpm
Temperature Range	-10°C to 40°C
Control Interface	Digital
Weight	400 kg
Refrigeration System	CFC-free
Power Requirements	200-240 V, 50/60 Hz

Summary

Revision History

Revision AE, December 2013

Added JA-14.50 rotor and related tables.

Revision AF, February 2014

Changed Polyallomer to polypropylene in several chapters.

Revision AG, October 2014

Added Avanti JXN-30, JXN-26 Centrifuges, and JA-18 Rotor.

Safety Notice

Alerts for Warning, Caution, Important, and Note

Explains the meaning of safety alert symbols and terms.

Chemical and Biological Safety

Discusses handling of chemical and biological hazards.

Safety Notice

Mechanical Safety

Covers safety precautions related to mechanical aspects of rotors and centrifuges.

Safety Notice

RoHS Notice

Explains RoHS compliance labels and their meaning.

Scope

Scope of this Manual

Details what the manual covers and how it should be used.

CHAPTER 1 Rotors

Introduction

Provides an overview of rotor types, selection, and operation.

General Description

Describes the basic characteristics and types of rotors.

Rotor Selection

Guides users on how to select the appropriate rotor for their application.

Materials

Discusses the materials used in the construction of rotors.

Drive Pins

Explains the function and importance of drive pins in rotor installation.

Rotor Types and Separations

Describes fixed-angle, swinging-bucket, vertical-tube, and rack-type rotors and their uses.

Pelleting (Differential Separation)

Explains how pelleting separates particles based on sedimentation coefficients.

Isopycnic and Rate Zonal Separations

Methods of particle separation based on density and sedimentation coefficient.

Blood Component Separations

Centrifugation as a method for processing blood components.

General Operating Information

General guidelines for operating centrifuges and rotors.

Rotor Balance

Explains how to balance rotor loads for stable operation.

Rotor Tie-Down

Describes devices and procedures for securing rotors to the drive spindle.

Overspeed Protection

Explains mechanisms to prevent rotors from exceeding their rated speeds.

Allowable Run Speeds

Discusses conditions that may require reducing maximum allowable rotor speeds.

Temperature Compensation

Explains the need for temperature compensation and how to set it.

Centrifuge Temperature Compensation

Provides instructions for setting temperature compensation on different centrifuge models.

CHAPTER 2 Tubes, Bottles, and Accessories

Introduction

Introduces labware used in Beckman Coulter J series rotors.

Labware Selection Criteria

Discusses factors influencing the choice of labware for centrifugation.

Labware Material Compatibility

Details chemical compatibility of labware materials with solvents and samples.

Labware Types

Describes various types of tubes, bottles, and accessories.

Capping and Sealing

Covers methods for capping and sealing tubes, bottles, and plates.

Sample Recovery

Guidelines for recovering samples from rotors, buckets, and carriers.

CHAPTER 3 Tubes, Bottles, and Accessories

Introduction

Contains general instructions for filling, capping, and sample recovery.

Gradient Preparation

Discusses methods for preparing density gradients using gradient formers.

General Filling and Sealing or Capping Requirements

Provides general requirements for filling and sealing tubes and bottles.

Working with Physiological Fluids

Discusses safety precautions when handling physiological fluids.

Filling Open-Top Tubes

Instructions for filling open-top tubes for various rotor types.

Capping Tubes

Details on capping tubes, including types of caps and requirements.

Filling and Capping Tubes

Instructions for filling and capping polycarbonate and polypropylene bottles.

Filling and Loading Cups in the JS-5.0 Rotor

Instructions for filling and loading labware cups in the JS-5.0 Rotor.

Filling and Sealing Quick-Seal Tubes

Step-by-step guide for filling and heat-sealing Quick-Seal tubes.

Methods for Sealing Quick-Seal Tubes

Procedures for sealing Quick-Seal tubes using different methods.

Capping Multiwell Titer Plates

Instructions for capping multiwell titer plates using cap strips or foil lids.

Using Adapters

General instructions for filling and capping tubes and bottles with adapters.

Using Aerosolve Canisters

Details the use of Aerosolve canisters to minimize aerosol leakage.

Using Blood Bag Cups

Instructions for loading blood bags into cups for centrifugation.

Making Ultra-Clear Tubes Wettable

Provides a method to make Ultra-Clear tubes wettable.

CHAPTER 4 Using Fixed-Angle Rotors

Introduction

Introduces the use of fixed-angle rotors in J series centrifuges.

Description

Describes the features and applications of fixed-angle rotors.

Rotor Preparation and Loading

Step-by-step instructions for preparing and loading fixed-angle rotors.

Operation

Details operating procedures for fixed-angle rotors.

Removal and Sample Recovery

Procedures for removing rotors and recovering samples.

CHAPTER 5 Using Swinging-Bucket Rotors

Introduction

Introduces the use of swinging-bucket rotors in J series centrifuges.

Description

Describes the features and applications of swinging-bucket rotors.

Rotor Preparation and Loading

Step-by-step instructions for preparing and loading swinging-bucket rotors.

Symmetric and Balanced Loading

Explains the importance and methods of symmetric and balanced loading of buckets/carriers.

Operation

Details operating procedures for swinging-bucket rotors.

Sample Recovery

Guidelines for recovering samples from swinging-bucket rotors.

CHAPTER 6 Using Vertical-Tube and Rack-Type Rotors

Introduction

Introduces the use of vertical-tube and rack-type rotors.

Description

Describes the features and applications of vertical-tube and rack-type rotors.

Vertical-Tube Rotors

Explains the function and use of vertical-tube rotors.

Rack-Type Rotors

Describes the function and use of rack-type rotors.

Rotor Preparation and Loading

Instructions for preparing and loading rack-type rotors.

Removal and Sample Recovery

Procedures for removing rack-type rotors and recovering samples.

CHAPTER 7 Care and Maintenance

Introduction

Provides information on rotor and accessory care, and troubleshooting.

Rotor Care

Covers regular cleaning, inspection, corrosion prevention, and lubrication of rotors.

Cleaning

Details cleaning procedures for rotors and their components.

Sterilization and Disinfection

Discusses methods for sterilizing and disinfecting rotors and components.

Inspection

Emphasizes the importance of regular inspection for rotor condition.

Lubrication

Explains lubrication procedures for rotor components like O-rings and pivot pins.

Diagnostic Hints

Lists common operating problems and their suggested solutions.

APPENDIX A Chemical Resistances for Beckman Coulter Centrifugation Products

List of Chemical Resistances

Refers to a separate document for chemical resistance data.

APPENDIX B Temperature Compensation Tables

Introduction

Introduces tables of temperature compensation units for various rotors.

Temperature Compensation

Explains the need for temperature compensation and how to set it.

APPENDIX C Gradient Materials

Introduction

Provides reference information on commonly used gradient materials.

APPENDIX D Blood Component Separation

Introduction

Provides an overview of blood separation procedures using Beckman Coulter J series centrifuges.

Blood Bank Collection Overview

Describes the composition of blood and the process of component separation.

Components and Typical Usage

Details common blood components and their typical applications.

Separation of Blood Components by Centrifugation

Explains centrifugation techniques for blood component separation.

Tips for Optimum Centrifugation Runs

Offers tips for safe and efficient blood component centrifugation runs.

APPENDIX E References

List of References

Lists documents and publications related to the manual's content.