Beckman Coulter Vi-Cell XR - Cmcresson

CMCresson 15-March-06
Parameter
Range
Default
Details
Images
1 – 100
50
Increase value for very low concentration samples
Size
3 - 70 µm
10 -40
µm
If a particular cell is being problematic, place
cursor over cell and size is displayed below
image
Brightness
75 – 90
85
Picks out objects from background
Increase includes more objects
Decrease includes less objects
Sharpness
60 – 200
100
Picks out objects from background
Decrease to include more objects
Increase to include less objects
Spot
Brightness
75 %
Determines cell viability
Increase value more cells counted as dead
Decrease value more cells counted as live
Spot Area
1 – 20 %
5 %
Ratio of the bright spot to total area
Determines cell viability
Increase value more cells counted as dead
Decrease value more cells counted as live
Minimum
Circularity
0–0.5
0
Excludes dead cells so they are considered debris
0 excludes no dead cells
1 excludes all dead cells
Decluster
None, low,
med, high
medium
Ability of software to pick individual cells from a
cluster of multiple cells
None counts a cluster as 1 cell