CFX96 and CFX384 Systems Manual
9
Running Experiments
Recommended Plastic Consumables
Run only 384-well plates in the CFX384 system. For optimal results, Bio-Rad recommends the
following consumables for the CFX384 system (catalog numbers are provided in bold):
• HSP-3805. Low-profile 384-well Hard-Shell® plates with clear shell and white wells
• HSP-3866. Low-profile 384-well Hard-Shell plates with black shell and white wells
• MSB-1001. Microseal ‘B’ adhesive seals, optically clear
The CFX96 system accepts both low profile 0.2 ml plates and tubes. For optimal results, Bio-
Rad recommends the following consumables:
• MLL-9601. Low-profile 96-well unskirted plates with clear wells
• MLL-9651. Low-profile 96-well unskirted plates with white wells
• HSP-9601. Hard-Shell 96-well skirted plates with white shell and clear wells
• HSP-9655. Hard-Shell 96-well skirted plates with white shell and white wells
• TLS-0801. Low-profile 0.2 ml 8-tube strips without caps, clear wells
• TLS-0851. Low-profile 0.2 ml 8-tube strips without caps, white wells
• TCS-0803. Optical flat 8-cap strips, for 0.2 ml tubes and plates
• MSB-1001. Microseal ‘B’ adhesive seals, optically clear
Loading the Block
To load your reactions in the block, follow these suggestions:
• Click the Open Lid button located on software’s Start Run tab (see “Start Run Tab”
on page 24), or press the lid button on the front of the system (Figure 1) to start
opening the motorized lid.
WARNING! The lid moves slowly at first, and then increases speed when it opens
or closes.
• Place the 0.2 ml microplate or tube strips with sealed lids in the block. Check that
the tubes are completely sealed to prevent leakage. For optimal results load sample
volumes of 10–25 μl for the CFX96 system and load sample volumes of 5–20 μl for
the CFX384 system.
NOTE: For accurate data analysis, check that the orientation of reactions in the
block is exactly the same as the orientation of the well contents in the software
Plate tab (see “Plate Tab” on page 23). If needed, edit the well contents before,
during, or after the run. As shown in Figure 11 on page 10, rotating the microplate
by 180
o
causes the software to expect the reading for well H3 to be an empty A10
well.
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