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General| Category | Thermal Cycler |
|---|---|
| Temperature Range | 4-99°C |
| Block Format | 48-well |
| Heating Rate | 3°C/s |
| Cooling Rate | 2°C/s |
| Block Type | Aluminum |
| Power Supply | 230 V, 50/60 Hz |
| Power Consumption | 600 W |
| Temperature Uniformity | ±0.3 °C (at 55 °C) |
Critical safety instructions for electrical hazards and proper usage, including voltage selector checks.
Explanation of symbols indicating high temperatures, explosive boiling, and ventilation requirements.
Identification of front panel components including display, keypad, power switch, and lid release.
Details of rear ports like RS-232, parallel printer, and fuse holder.
Explanation of the graphical display, function keys, and cursor navigation for operation.
The instrument's self-test sequence upon startup, displaying serial number and software version.
Information conveyed by the instrument's operational display regarding program status.
Procedures for closing, opening, and adjusting the heated lid for optimal pressure.
Step-by-step guide to unlock the lid mechanism if it becomes stuck or arrested.
Choosing storage locations for PCR programs, including main and subdirectories.
Options for saving programs in the main directory or individual subdirectories.
Process for assigning descriptive names to program storage subdirectories.
Assigning descriptive names to individual PCR programs for easier retrieval.
Configuring the temperature for the instrument's heated lid, recommended 10°C above denaturation.
Enabling or disabling the lid pre-heating function to prevent condensation.
Inputting temperature, time, gradient, and optimization values for each PCR step.
Setting up temperature variations across the block for reaction optimization.
Checking the actual temperatures across block rows during gradient use.
Defining the total number of cycles and backloop repetitions for a program.
Programming temperatures below ambient conditions for specific applications.
Finalizing and storing created PCR programs in the instrument's permanent memory.
Modifying individual steps within a PCR program by deleting or inserting new steps.
Duplicating existing programs to different storage locations or directories.
Removing unwanted programs from the instrument's memory.
Adjusting time per cycle to compensate for enzyme activity loss.
Gradually decreasing annealing temperature across cycles for optimization.
Modifying the speed of temperature changes between steps for compatibility.
Procedure for choosing and initiating a PCR run from stored programs.
Information conveyed by the instrument's operational display during a run.
Checking the estimated time left for the current program execution.
Real-time view of temperatures across the block during gradient runs.
How to temporarily halt or permanently stop an active program.
Options for printing program data, graphic protocols, and all programs.
Enabling or disabling audible signals for program events like pause or finish.
Switching the instrument's interface language between English and German.
Information on PC control via RS232 interface using Tcontrol iT software.
Configuring the instrument for different block module types (e.g., 48-well, 96-well).
Guidelines for cleaning and maintaining the instrument's housing.
Instructions for removing and installing different block modules using hexagonal keys.
Diagnosing and resolving issues with slow temperature ramps due to fan inlet blockage.
Handling restarts due to power interruptions or fluctuations and avoiding shared power sources.
Detailed technical data and specifications of the TGRADIENT 96.
Part numbers and details for ordering the cycler and compatible modules.
Procedures for safely returning the instrument for service, including decontamination.
Terms and conditions of the instrument's warranty coverage.
Form required for certifying equipment decontamination before handling or repair.
