BRIGHT OTF 5000 - Knife Holder; Thin Section Conversion Kit

OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012
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2.1.6 /D - CRYOMATIC SPECIMEN TEMPERATURE CONTROL (where
applicable)
FIG 8
To set the specimen temperature, press and release the Set
button and then press the Up or Down buttons until the desired
temperature is set. After 5 seconds the actual temperature will
show. To review the Set temperature at any time press and
release the Set button.
NB: This system can only set temperatures above the selected
chamber temperature, not colder.
2.1.7 QUICK FREEZER PLATE
FIG 9
Remove the frost elimination cover before using the quick freezer plate.
This plate runs at or below the set chamber temperature (typically 10ºC
below chamber temperature) and is useful for storage or pre-cooling of
knives etc. Although it is possible to freeze fresh tissue on the quick
freezer plate, it is generally preferable to use a much colder medium.
The temperature of the quick freezer plate is displayed constantly on
the main control panel. Do not forget to replace the frost elimination
cover after using the quick freezer plate, this helps prevent a build up of
frost.
2.1.8 /QF - SPECIMEN QUICK FREEZER (where
applicable)
FIG 10
The specimen quick freezer (/QF) is
designed for rapid freezing of specimens
mounted on specimen holders. Cooled
by a separate compressor, the /QF can
either run continuously or be switched
on 30 minutes before use. Please note
that the operation of the freezer is
dependant on the operation of the
chamber compressor. The /QF will not
function when a chamber temperature
warmer than -15ºC is selected. Optimum
performance is achieved with chamber
temperatures of -20ºC or colder.
SAFETY: The quick freezer operates at extremely low temperatures and may cause freeze
burns. Suitable protective gloves should be worn when handling frozen specimen holders.
Switching On
1 Ensure that the /QF is clean and dry before switching the cryostat on, as the cold chamber
temperature will freeze the quick freezer even when it is switched off.
2 Ensure that the appropriate sleeve is fitted into the freezer block to accept the object holder
to be used, (a sleeve for the standard 22mm object holder is fitted and a sleeve for the
37mm object holder is supplied in addition).