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OTF, enables tissue samples to be rapidly frozen inside the cryostat chamber to -80ºC. In most
cases this will avoid the need for external freezing media.
3.1.5 BRIGHT CLINI-RF
The Bright Clini-RF is a small, free standing, top opening, ultra low
temperature freezer. Its function is to rapidly freeze tissue specimens
ready for cryotomy or for storage. Inside the Clini-RF are two cylindrical
chambers, the upper chamber is held at minus 40ºC and the lower at
minus 80ºC. It is an ideal replacement for CO
2
freezing techniques.