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BRIGHT OTF 5000 - User Manual

BRIGHT OTF 5000
48 pages
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Model shown HS
Serial No:
St Margarets Way Stukeley Meadows Industrial Estate Huntingdon Cambridgeshire England PE29 6EU
Tel: +44 (0) 1480 454528/451980/451499 . Fax: +44 (0) 1480 456031
Email: sales@brightinstruments.com . Website: www.brightinstruments.com
INSTRUCTION MANUAL
OTF 5000 All Models
BS EN ISO 9001:2008
Certificate No. Q 05456

Table of Contents

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Do you have a question about the BRIGHT OTF 5000 and is the answer not in the manual?

Summary

Safety Information

Consumer Protection

Legal information regarding product liability and safe handling.

Electrical Safety

Safety precautions related to electrical components and connections.

Microtome Knives and Blades Safety

Critical safety warnings for handling sharp microtome knives and blades.

Safe Operation Guidelines

Safety guidelines for operating the microtome safely.

Low Temperatures and Warnings

Accessory Safety

Information on accessory fluids and their safe laboratory use.

Product Safety Suggestions

How to submit suggestions for improving product safety.

Decontamination Certificates

Procedures and requirements for returning instruments for decontamination.

General Warning

General warning before starting operating instructions; use by competent persons.

Warranty and Initial Setup

Receipt and Unpacking

Instructions for inspecting the instrument upon receipt and unpacking.

Assembly and Installation

Fitting the Handwheel

Step-by-step guide to correctly install the microtome handwheel.

Drain Tube and Plug

Instructions for installing the drain tube and plug for water removal.

Installation of Trays and Shelves

Guide on how to install internal trays and shelves within the chamber.

Frost Elimination Cover

How to place the frost elimination cover on the quick freezer plate.

Microtome Installation and Removal

Detailed instructions for safely installing and removing the microtome unit.

Insulated Night Plug

Instructions for unpacking and storing the insulated night plug.

Thin Section Conversion Kit

Information on installing the thin section conversion kit.

Ultra Violet Light Decontamination Unit

Instructions for using the UV light decontamination unit.

Glove Port

How to access the cryostat chamber using the glove port.

Foot Switch

Instructions for connecting and using the foot switch.

Positioning

Guidelines for correctly positioning the cryostat for optimal ventilation and operation.

Electrical Setup and Safety

Settling Procedure

Recommended settling time for the cryostat before initial power-on.

Electrical Requirements

Specifies power supply requirements and wiring connections for safe operation.

Electrical Safety Precautions

Crucial safety points regarding earthing and electrical connections.

Operating Instructions

Introduction

Overview of the OTF5000 cryostat and its features.

Switching On

Steps for powering on the instrument after settling and initial setup.

Cabinet Controls

Mains Switch

Controls the main power supply to the cryostat.

Light Switch Button

Activates the internal fluorescent lamp for illumination.

Defrost Switch

Initiates or works in conjunction with the defrost cycle.

Defrost Clock

Sets the timing for automatic evaporator defrost cycles.

Chamber Temperature Control

Setting and monitoring the internal chamber temperature for optimal sectioning.

Cryomatic Specimen Temperature Control

Controls and displays specimen temperature for advanced applications.

Quick Freezer Plate

Information on the quick freezer plate for pre-cooling or freezing.

Specimen Quick Freezer

Details on the optional rapid specimen freezing unit.

Dual Refrigeration System

Information on the dual refrigeration system for enhanced cooling.

Motorised Advance;Rewind

Operation of the motorised system for specimen advance and rewind.

Handwheel Operation

Manual operation of the microtome using the handwheel.

Motor Drive

Operation of the motor drive for enhanced control and speed.

Electronic Section Counter

Features and operation of the electronic section counting system.

Foot Switch

Operation of the foot switch for hands-free control.

Ultra Violet Light Decontamination Unit

Information on the UV light unit for chamber decontamination.

Glove Port

How to access the cryostat chamber using the glove port.

Microtome Controls - Standard Knife Block

Knife Guards

Safety feature; instructions for use and adjustment of knife guards.

Knife Holder

How to install, clean, and adjust the knife holder for optimum performance.

Thin Section Conversion Kit

Steps to fit the thin section conversion kit for finer sectioning.

Anti-Roll Assembly Setup

Detailed guide on setting up the anti-roll plate for better section quality.

Microtome Controls - Quick Release Feather Blade Holder

Blade Guards

Safety instructions for fixed and wire blade guards.

Blade Holder Operation

How to install, adjust, and remove feather blades.

Anti-Roll Assembly Setup

Setting up the anti-roll plate for optimal section collection.

Common Microtome Controls

Coarse Advance Control

How to perform large movements for specimen trimming.

Section Thickness Control

Setting the desired section thickness and resetting the control.

Advance; Rewind Control

Mechanism for specimen advance and rewind, including feed indicator.

Left Hand Side Advance;Rewind Control

Operation of the left-hand side advance/rewind control.

Specimen Holder

How to fit and orient specimens using the vice and holders.

Cutting and Collecting Sections

Basic Freezing Techniques

Methods for freezing tissues, including LN2, CO2, and freezer plates.

Liquid Nitrogen Freezing

Using liquid nitrogen as a freezing agent for tissues.

Carbon Dioxide Gas Freezing

Freezing tissues using carbon dioxide gas.

Carbon Dioxide Solid Freezing

Freezing tissues using solid carbon dioxide (dry ice).

Freezer Plates

Information on the standard and optional quick freezer plates.

Bright Clini-RF Freezer

Description of the Clini-RF freezer as an alternative.

Fitting the Specimen

Step-by-step guide to preparing and fitting specimens for sectioning.

Trimming the Specimen

How to trim the specimen block to prepare it for sectioning.

Section Cutting and Collection

Procedures for cutting sections and collecting them onto slides.

Hints on Sectioning

Tips and advice for improving sectioning quality and technique.

Frozen Sectioning Problem Solving

Solutions Check List

A checklist of common factors to examine when troubleshooting sectioning issues.

Specific Problems

Detailed solutions for common sectioning problems like thickness and crumbling.

Sections Curling Over Anti-Roll Plate

Troubleshooting steps for sections curling over the anti-roll plate.

Sections Curling Under Anti-Roll Plate

Troubleshooting steps for sections curling under the anti-roll plate.

Sections Sticking to Anti-Roll Plate

Troubleshooting steps for sections sticking to the anti-roll plate.

Sections Curling After Lifting Anti-Roll Plate

Troubleshooting steps for sections curling after the anti-roll plate is lifted.

Sections Failing to Flatten

Troubleshooting steps for sections not flattening or passing correctly.

Cutting Temperatures

Fresh, Unfixed Tissue

Recommended cutting temperatures for fresh and unfixed tissue types.

Fixed Tissue

Recommended cutting temperatures for fixed tissues.

Daily Care and Maintenance

Automatic Defrost Cycle

How the automatic defrost cycle functions to clear evaporator frost.

Total Defrosting Procedure

Procedure for performing a complete defrost for cleaning or maintenance.

Microtome Knives Handling

Essential safety and handling guidelines for microtome knives.

Operating in Extreme Conditions

Servicing and Repairs

Service Procedures (UK)

Contact information and procedures for service within the UK.

Service Procedures (Rest of World)

Contact information and procedures for service outside the UK.

Simple Servicing Procedures

Changing Fuses

Step-by-step guide to replacing blown fuses.

Changing Fluorescent Tube

Instructions for replacing the internal light tube.

Decontamination

Accessories

Object Holders

Description of various object holders available for the microtome.

Quick-Release Type Holders

Overview of quick-release object holder systems.

Orientating Specimen Holders

Details on orientating specimen holders for precise positioning.

Knives

Lever Release Feather Blade Holder

Magnaplate, Anti-Roll Plate

Magnacut, Magnetic Disposable Blade System

Easi-Set, Anti-Roll Plates

Feed Screws

Cryospray

Cryo-M-Bed

Spare Parts

5040 Parts Reference

Reference to appendix for 5040 model parts.

5040 Electronics Spare Parts

List of spare electronic parts for the 5040 model.

Accessories and Consumables

Product Information

Introduction

General introduction to the OTF5000 cryostat.

Special Features

Highlights key features and benefits of the OTF5000.

Configurations Overview

Details the different available configurations of the OTF5000 cryostats.

Basic Specification Details

Detailed technical specifications for the Basic model.

LS Specification Details

Detailed technical specifications for the LS model.

HS Specification Details

Detailed technical specifications for the HS model.

BRIGHT OTF 5000 Specifications

General IconGeneral
Maximum Temperature1200°C
Tube Diameter50mm
Power Supply220V, 50/60Hz
Temperature ControlPID Controller
Thermocouple TypeK-type
TypeTube Furnace
Heating Rate20°C/min
Cooling Rate10°C/min
AtmosphereAir

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