BRIGHT OTF 5000 - Assembly and Installation; Fitting the Handwheel; Drain Tube and Plug; Installation of Trays and Shelves

OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012
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2.1.3 DEFROST SWITCH (Located on the side panel) 9
2.1.4 DEFROST CLOCK (Located on side panel) 9
2.1.5 CHAMBER TEMPERATURE 10
2.1.6 /D - CRYOMATIC SPECIMEN TEMPERATURE CONTROL (where applicable) 11
2.1.7 QUICK FREEZER PLATE 11
2.1.8 /QF - SPECIMEN QUICK FREEZER (where applicable) 11
2.1.9 /EC or /LT or /LOWEC - DUAL REFRIGERATION SYSTEM (where applicable) 12
2.1.10 MOTORISED ADVANCE/REWIND (where applicable) 13
2.1.11 HANDWHEEL 13
2.1.12 /MR or /MR(S) - MOTOR DRIVE (where applicable) 14
2.1.13 /C - ELECTRONIC SECTION COUNTER (where applicable) 14
2.1.14 /V - FOOT SWITCH (where fitted) 14
2.1.15 /U - ULTRA VIOLET LIGHT DECONTAMINATION UNIT (where applicable) 15
2.1.16 /Y - GLOVE PORT (where applicable) 15
2.2 MICROTOME CONTROLS - STANDARD KNIFE BLOCK 16
2.2.1 KNIFE GUARDS 16
2.2.2 KNIFE HOLDER 17
2.2.3 /X - THIN SECTION CONVERSION KIT (where applicable) 17
2.2.4 ANTI-ROLL ASSEMBLY 18
2.3 MICROTOME CONTROLS - Quick Release Feather Blade Holder 20
2.3.1 BLADE GUARDS 21
2.3.2 BLADE HOLDER 21
2.3.3 ANTI-ROLL ASSEMBLY 21
2.4 Common MICROTOME CONTROLS 23
2.4.1 COARSE ADVANCE CONTROL 23
2.4.2 SECTION THICKNESS CONTROL 23
2.4.3 ADVANCE / REWIND CONTROL 23
2.4.4 SPECIMEN HOLDER 24
2.5 ALTERNATIVE FEED SCREWS 24
3 CUTTING & COLLECTING SECTIONS 25
3.1 BASIC FREEZING TECHNIQUES 25
3.1.1 LIQUID NITROGEN 25
3.1.2 CARBON DIOXIDE - GAS 25
3.1.3 CARBON DIOXIDE - SOLID 25
3.1.4 FREEZER PLATES 25
3.1.5 BRIGHT CLINI-RF 26
3.2 FITTING THE SPECIMEN 27
3.3 TRIMMING THE SPECIMEN 28
3.4 SECTION CUTTING AND COLLECTION 28
3.5 HINTS ON SECTIONING 28
3.6 FROZEN SECTIONING - A PROBLEM SOLVING GUIDE 29
3.6.1 SOLUTIONS - CHECK LIST 29
3.6.2 SPECIFIC PROBLEMS 29
3.7 CUTTING TEMPERATURES 30
3.7.1 FRESH, UNFIXED TISSUE 30