Buchi Pure C-810 - Page 100

10 | Appendix
Büchi Labortechnik AG
100/102
Operation Manual Pure Chromatography Instruments
The LC-Transfer tool converts an HPLC gradient into a Flash Chromatography
gradient.
Specification of the sample needed:
 One run on a HPLC column (in gradient elution mode)
 Times %B changes (t1, t2 and t3)
Precondition:
R The display shows the dialog Navigator . See Chapter10.3.1 "Open the
navigator", page97
u Select the LC-Transfer tab.
u Select the HPLC column type used from the drop-down list.
u Enter the flow rate used in the HPLC separation.
u Enter the following HPLC gradient conditions:
 lower and higher %B
 times t1, t2 and t3
u Select the cartridge you wish to use for the Flash separation from the drop-down
list.
ð The cartridge is selected.
ð The display shows the default flow rate.
u Adjust the flow rate according to your needs.
u Tap the button [Calculate].
ð The display shows the results.
u Adjust the following values if necessary:
 Flow rate
u Tap the button [Accept].
ð The gradient is saved for a run.
ð The dialog box closes.
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