Buchi Pure C-810 - Page 99

Büchi Labortechnik AG
Appendix | 10
Operation Manual Pure Chromatography Instruments
99/102
Specification of the samples needed:
 Two isocratic runs on a HPLC column
 Different mobile phase solvent concentrations
 Retention times (t1 and t2) of the components from the chromatograms.
Precondition:
R The display shows the dialog Navigator . See Chapter10.3.1 "Open the
navigator", page97
u Select the LC-C18 tab.
u Select the HPLC column used from the drop-down list.
u Select the weaker solvent at drop-down list A.
u Select the stronger solvent at drop-down list B.
u Enter the solvent concentrations used in the HPLC separations.
u Enter the retention times for each separation under each chromatograph.
u Select the column you wish to use from the drop-down list
ð The column is selected.
ð The display shows the default flow rate.
u Adjust the flow rate according to your needs.
u Select the radio button for Speed or Purity.
u Tap the button [Calculate].
ð The display shows the results.
u Tap the button [Accept].
ð The gradient is saved for a run.
ð The dialog box closes.
10.3.4 LC-Transfer
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