10 | Appendix
Büchi Labortechnik AG
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Operation Manual Pure Chromatography Instruments
u Measure the distance the spot moved on the silica TLC plates.
u Divide the measured value by the distance the solvent traveled.
ð This is your Rf- value.
u Enter the value in the Rf entry field.
u Select the column you wish to use from the drop-down list
ð The column is selected.
ð The display shows the default flow rate.
u Adjust the flow rate according to your needs.
u Select the radio button for Speed or Purity.
u Tap the button [Calculate].
ð The display shows the results.
u Adjust the following values if necessary:
Column
Flow rate
Speed / Purity
u Tap the button [Accept].
ð The gradient is saved for a run.
ð The dialog box closes.
10.3.3 LC-C18
The LC-C18 Tool uses isocratic HPLC separation data to provide a recommended
gradient for reversed phase separations of 2 components.
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