Buchi Pure C-810 - Finding Separation Conditions with Navigator

Büchi Labortechnik AG
Appendix | 10
Operation Manual Pure Chromatography Instruments
97/102
10.3 Finding out separation conditions with the navigator
10.3.1 Open the navigator
Navigation path
➔ Gradient panel
u Navigate to the Gradient panel according the navigation path.
u Tap the button [Navigator].
ð The display shows the dialog Navigator .
10.3.2 TLC Silica
The TLC-Silica tool uses TLC separation data to provide a recommended gradient for
silica separations of 2 or 3 components.
Specification of the samples needed:
 Two different solvent concentrations
 Two separations
 Silica TLC plates
Precondition:
R The display shows the dialog Navigator . See Chapter10.3.1 "Open the
navigator", page97
u Select the TLC- Silica tab.
u Select the radio button for two or three components.
u Select the weaker solvent at drop-down list A.
u Select the stronger solvent at drop-down list B.
u Enter the solvent B concentrations used in the TLC separations.
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