Solution Preparation
39. Fill the bottles for the bottle stirrer with the contents of
a SPRINTPAK (sold separately) as follows:
a. Pour deionized water into the empty 1-liber bottle
labeled Solution 3, leaving suffi cient space to add
the solution. Pour Solution 3 into the bottle.
b. Pour deionized water into the empty 1-liter bottle
labeled Solution 2, leaving suffi cient space to add
the solution. Pour Solution 2 into the bottle.
Note: Adding deionized water prior to adding the concen-
trated solutions aids in preventing bubbling during solution
addition.
CAUTION
During iTAG® Solution 1 preparation, ensure that
the 4-liter bottle is empty. Because each iTAG®
mixture has a specifi c standard, mixing iTAG® So-
lution 1 from two (2) SPRINTPAKs will render the
solution unusable.
c. Prepare the iTAG® Solution 1 by one of the fol-
lowing methods.
Gravimetric Method (CEM Recommended)
i. Remove any remaining iTAG® Solution 1 from
one of the 4-liter bottles and rinse it thoroughly
with deionized water.
ii. Place the 4-liter bottle and a funnel on a top-
loading balance (minimum 3.5 kg capacity).
iii. Press the “Tare” button on the balance.
iv. Add approximately 500 grams of deionized
water to the 4-liter bottle.
v. Pour the iTAG® Solution 1 into the 4-liter bottle.
vi. Rinse the iTAG® Solution 1 bottle with deion-
ized water and add the solution to the 4-liter
bottle. Repeat this step until the rinse water is
clear.
vii. Fill the 4-liter bottle with deionized water until a
total weight of 2514 grams is reached. Proceed
with step d.
Volumetric Method
i. Pour approximately 250 mL of deionized water
into a graduated cylinder.
ii. Pour the iTAG® Solution 1 into the cylinder.
5
iii. Rinse the iTAG® Solution 1 bottle with deion-
ized water and add the solution to the cylinder.
Repeat this step until the rinse water is clear.
iv. Fill the cyllinder with deionized water until a
total volume of 1000 mL is reached.
v. Remove any remaining iTAG® Solution 1 from
one of the 4-liter bottles and rinse it thoroughly
with water.
vi. Using a funnel, pour the mixture from the
graduated cylinder into the 4-liter bottle.
vii. Pour an additional 1000 mL of deionized water
into the graduated cylinder. Add this water to
the 4-liter bottle.
viii. Pour an additional 500 mL of deionized water
into the graduated cylinder. Add this water to
the 4-liter bottle.
ix. Proceed to step d.
Standard Method
i. Pour approximately 1 liter of deionized water
into one of the 4-liter bottles.
ii. Add iTAG® Solution 1 from the SPRINTPAK
into the bottle.
iii. Rinse the iTAG® Solution 1 bottle with deion-
ized water and pour the water into the 4-liter
bottle.
iv. Fill the 4-liter bottle to the line indicated on the
bottle label with deionized water (2.5 liters total
volume).
d. Place a stir bar in the iTAG® Solution 1 bottle.
Note: The magnetic stir bar must be between 35 - 45 mm
long and a maximum of 20 mm wide. It must be “egg”
shaped.
e. Permit this solution to sit for at least fi fteen (15)
minutes prior to use to allow any bubbles and/or
gas to dissipate.
Note: Do not use any iTAG® Solution 1 from a previous
SprintPak.
Note: If the solution contacts hands, wash hands with
soap and water.
f. Place the lids on the bottles.
g. Agitate (swirl vigorously) the iTAG® Solution 1
bottle to ensure that the solution is thoroughly
mixed with the deionized water.
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