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Fluid Imaging Technologies FlowCam 8000 series User Manual

Fluid Imaging Technologies FlowCam 8000 series
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FlowCam
®
8000 Series
Dynamic Imaging Particle Analyzer
User Guide
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Fluid Imaging Technologies FlowCam 8000 series Specifications

General IconGeneral
BrandFluid Imaging Technologies
ModelFlowCam 8000 series
CategoryMeasuring Instruments
LanguageEnglish

Summary

Warnings and Safety Precautions

Laser Precautions

Precautions related to the Class 1 laser product within the FlowCam 8400 analyzer housing to prevent exposure.

Electrical and Mechanical Cautions

Safety warnings regarding electrical shock, unexpected movement of the syringe pump/barrel, and equipment damage.

Introduction to FlowCam Analysis

System Overview

Describes the FlowCam 8000 Series Dynamic Imaging Particle Analyzer and its core functions.

Technical and Physical Specifications

Analyzer Component Identification

FlowCam System Operation

Key Operational Components

Details the major components and their functions within the FlowCam system, including the syringe pump, camera, and software.

Optical Configurations and Fluorescence

Objectives and Flow Cells

Explains the different objective lenses and flow cell options available for particle measurement.

Fluorescence Detection System

Details the fluorescence system, including lasers, optics, and PMT detectors for particle analysis.

Getting Started: Welcome

Package Contents

Lists the items included in the FlowCam analyzer shipping container.

Installation Environment

Provides guidelines for selecting an optimal location and power supply for the FlowCam analyzer.

Computer Peripherals Setup

Instructions for unpacking and connecting the necessary computer peripherals like monitor, keyboard, and mouse.

Setting Up the FlowCam Analyzer

Connecting Peripherals

Details how to connect keyboard, mouse, and monitor to the FlowCam analyzer's ports.

Powering On the Analyzer

Step-by-step guide for powering on the FlowCam analyzer and initiating the startup process.

Preparing the Analyzer for Use

Checking Internal Connections

Instructions to inspect tubing connections within the analyzer for proper seating and security.

Adding Analysis Solutions

Guide on filling the cleaner and rinse bottles with appropriate solutions for analysis.

Flow Cell Installation Guide

Flow Cell Handling and Cleaning

Precautions and procedures for handling, cleaning, and connecting the flow cell to prevent damage.

Flow Cell Installation Steps

Objective Lens Installation

Instructions for installing and changing objective lenses on the FlowCam analyzer.

Objective Lens Setup

Changing Objective Magnification

Procedure to change the objective lens magnification via the VisualSpreadsheet software.

Autofocusing the Flow Cell

Autofocus Beads and Procedure

Details the types of autofocus beads and the steps to perform the automatic focusing routine.

Autofocus Procedure Details

Flow Cell Positioning and Alignment

Guidance on positioning and aligning the flow cell using the positioning knob for optimal autofocus.

Manual Flow Cell Focusing

Manual Focus Procedure

Steps for manually focusing the flow cell using the Setup and Focus tool.

Manual Focusing Steps

Flow Cell Alignment for Manual Focus

Guidance on aligning the flow cell for manual focus using positioning controls and visual cues.

Flow Control for Manual Focus

Using Pump Control for Focusing

Instructions on using the Pump Control dialog to adjust flow rate and achieve clear particle images for focusing.

Defining Context Settings

Context Dialog and Tabs

Introduction to the Context dialog and its various tabs for configuring analysis parameters.

Context Settings: Capture Parameters

Particle Capture Configuration

Details settings within the Capture tab for defining particle capture parameters like distance and image saving options.

Context Settings: Flow Cell and Fluidics

Flow Cell Type Selection

How to select the appropriate flow cell type in the Context dialog.

Fluidics Configuration

Settings for sample volume, flow rate, autoimage rate, and priming within the Fluidics tab.

Context Settings: Filter, Reports, and Notes

Filter and Report Options

Configuration of image filters, report generation, and classification settings.

Notes and Stop Conditions

Using the Notes tab for comments and defining stop conditions for analysis termination.

Context Settings: Stop Conditions and Fluorescence

Stop Conditions Configuration

Setting parameters to automatically stop the analysis based on particle count, time, or volume.

Fluorescent Measurement Settings

Setting thresholds for particle acquisition and rejection in the FlowCam 8400 analyzer.

Performing a Sample Analysis

Analysis Preparation and Execution

Steps to prepare for sample analysis, including setting stop conditions and running the analysis.

Cleaning and Rinsing the Analyzer

System Flushing Procedure

Procedure to flush the FlowCam system using the pump's flush feature.

Manual Cleaning and Rinsing

Steps for manually cleaning and rinsing the FlowCam analyzer with cleanser and rinse solution.

Automatic Clean and Rinse Cycles

How to set up automatic clean and rinse cycles at the end of sample analysis.

Automatic Cleaning and Power Down

Configuring Automatic Cleaning Cycles

Setting up automatic clean and rinse cycles to run at the end of an analysis.

Powering Down the FlowCam Analyzer

Procedure for safely shutting down the FlowCam analyzer and its components.

Care and Maintenance Procedures

General Maintenance Guidelines

General guidelines for cleaning and rinsing the FlowCam analyzer after use.

Flow Cell Maintenance

Detailed instructions for cleaning and maintaining the flow cell, including specific warnings and best practices.

Fluidics Bottles and Tubing Maintenance

Maintenance checks for fluidics bottles and tubing connections to ensure proper function and integrity.

Analyzer Exterior Maintenance

Maintenance procedures for the main analyzer unit, including cleaning the exterior.

Appendix A: Pump Syringe Replacement

Procedure for Changing Pump Syringe

Using the Pump Controller for Syringe Change

Steps to use the Pump Controller dialog to initiate and complete a pump syringe change.

Selecting New Pump Syringe Size

Cleaning and Storing Old Syringe

Instructions for cleaning the previous syringe and replacing it in its container.

Appendix B: Frequently Asked Questions

Flow Cell Cleaning and Refocusing

Answers to common questions about cleaning clogged flow cells and refocusing after objective changes.

Analyzer Calibration and Fluid Levels

Addresses questions on analyzer calibration frequency and resetting fluid bottle levels.

Appendix C: Services and Support

FlowCam Training Opportunities

Describes four types of training opportunities offered for FlowCam users.

Preventive Maintenance Service

Details the benefits and components of a preventive maintenance service for the FlowCam analyzer.

Installation, Qualification, and Laboratory Services

Installation and Operational Qualification (IOQ)

Explains the process and requirements for IOQ services for the FlowCam analyzer.

Laboratory Services Offered

Outlines laboratory services including particle analysis and method development.

Standard Factory Limited Warranty

Flow Cell Limited Warranty

Customer Support Contact Information

Provides contact details for customer support via email, phone, fax, and mail.

Declaration of Conformity

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