6
Select the instruction AccumulatorWash in the Frac list.
7
Select the number of strokes to be used for washing with the parameter Strokes.
8
Click on the Execute button.
Setting delay volume
The delay volume between the UV flow cell in the chromatographic system and Fraction
Collector Frac-950 must be known to UNICORN. The delay volume is used to adapt the
collected fractions to the event marks generated by UNICORN.
1
In UNICORN, select menu System Control:System:Settings
2
Click the Specials radio button and select instruction FracParameters. The DelayVol
instruction becomes highlighted.
3
To change the setting, click on the up and down arrows for the DelayVol parameter,
or type a new value directly in the parameter window.
4
The appropriate value for your ÄKTA system is found in the ÄKTA design Optional
Configuration User Manual.
5
Click on the OK button. The value entered will be used until a further change is made.
44 Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC
4 Operation
4.3 Fraction Collector Frac-950
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