Flow control during tube change
The liquid flow during tube change can be handled in three different ways.
1
In UNICORN, select menu System Control:System:Settings.
2
Click the Specials radio button and select instruction FracParameters. Highlight the
TubeChange instruction.
3
For the parameter TubeChange, select one of the following options:
•
Tube
No synchronization of collection. Spillage will occur between tubes.
•
DropSync
Tube change is synchronized to drop release to minimize spillage.
Use i.d. 0.75 mm tubing between the DropSync and the accumulator.
•
Accumulator
During tube change, the flow is diverted to the accumulator which stores the
liquid. When the new tube is in position, the liquid is pressed out rapidly for col-
lection.
4
Flow rate limit recommendations using DropSync without spillage are given in the
table below. For higher flow rates the accumulator is recommended for spillage free
fractionation. For the 30 mm rack and Prep mode racks the accumulator is recom-
mended.
Flow rate limit using dropSync
[ml/min]
Rack type
0 to 1.0microplates
0 to 1.512 mm
0 to 2.018 and 30 mm
Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC 45
4 Operation
4.3 Fraction Collector Frac-950
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