Hettich UNIVERSAL 320 - Software: Parameters and Programming

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Rev.: 04 / 11.2023
AB1401en_SA
Software operation
7 Software operation
7.1
Centrifugation parameters
7.1.1 Relative centrifugal force, RCF
The relative centrifugal force RCF is dependent on the speed and the centri-
fuging radius.
The relative centrifugal force RCF is stated as a multiple of the acceleration
due to gravity (g).
The relative centrifugal force RCF is a dimensionless numerical value and is
used to compare the separation and sedimentation performance.
RCF = Relative Centrifugal Force
RPM = speed
r = centrifuging radius in mm = distance from the centre of the axis of
rotation to the bottom of the centrifuge tube.
7.1.2 Centrifugation of substances or mixtures of substances with a density higher than
1.2 kg/dm
3
The density of the substances or mixtures of substances must not exceed
1.2kg/dm³ during centrifugation at maximum speed. The speed must be
reduced for substances or substance mixtures with a higher density. The
permissible speed can be calculated using the following formula:
For example: Maximum speed 4000 RPM, density 1.6kg/dm³
If, in exceptional cases, the maximum load indicated on the bucket is
exceeded, the speed must also be reduced. The permissible speed can
be calculated using the following formula:
For example: Maximum speed 4000RPM, maximum load 300g, actual
load 350g
Please contact the manufacturer if you are not sure.