Human Humalyzer 2000 - Cleaning the Flowcell

Rev. B 15
Cleaning the Flowcell |
The flowcell should be cleaned when the instrument will not be used for an extended period, e.g.
overnight, end of shift, and when storing the flowcell. Proper cleaning will help to prevent
clogging of the flowcell tubing and valve tubing. Cleaning is extremely important to obtaining
accurate, repeatable results. If reagent, serum, or other proteinaceous fluid is allowed to dry in
the flowcell, it is extremely difficult to remove and its presence can affect test results.
To clean the flowcell:
1. Purge with air for at least 5 seconds.
2. Aspirate several ml of flowcell cleaning solution. Allow the solution to remain in the
flowcell for 3 minutes.
3. Aspirate 15 ml of distilled water then purge with air for 5 seconds.
一 4. Aspirate 0.1N sodium hydroxide (NaOH ). Allow the solution to remain in the flowcell
for 3 minutes.
5. Purge with air for at least 5 seconds.
6. Aspirate 0.1N hydrochloric acid (HCI). Allow the solution to remain in the flowcell for
3 minutes.
7. Purge with at least 15 ml of deionized water.
8. If the flowcell is to be removed for storage,, purge with air until no more fluid can be
seen flowing into the waste bottle. Otherwise, leave the flowcell filled with water.
Humalyzer 2000 Service Manual