Human Humalyzer 2000 - Temperature Calibration

34
Rev. B
Temperature Calibration
Materials and equipment:
“range message.
12
mm
borosilicate
tube
/
Reference
thermistor
probe
YSI
Inc.
#44108
or
equivalent.
30K
(Y
25°C,+
0.2°
interchangeability
Humalyzer
2000
Temperature
Calibration
Fixture
Digital
ohmmeter
(DMM),
0.5%
or
better
accuracy.
Ensure that the flowcell is active. Press CELL. Press YES to activate the flowcell.
Press CLEAR twice to revert to the temperature display.
Place a 12 mm borosilicate test tube filled with 1 ml of water into the incubation block.
Use a station near the center of the block. Suspend the reference thermistor probe in
the approximate center of the tube.
Remove the flowcell and install the Temperature Calibration Fixture into the read well.
Allow 15 minutes for equilibration.
Set the DMM to read 20K. Read the resistance of the thermistor and the fixture. If the
resistance is between 17724 Q and 18712 Q, no adjustments are needed. Otherwise,
continue to the next step.
Calculate the temperature offsets using the following formula:
T=R-18210
С
Where:
R is the measured resistance.
C is equal to 750 if the measured resistance is greater than 18210.
C is equal to 720 if the measured resistance is less than 18210.
Select test #210. The display shows:
Block adjust=
Type the offset calculated above for the block and press ENTER.
The display shows:
Cell adjust=
Type the offset calculated for the cell and press ENTER. The maximum allowed adjust-
ment is +/-1.8°C . Entering values that exceed this net number will produce an out of
“Humalyzer 2000 Service Manual