Human Humalyzer 2000 - Flowcell Tubing Replacement

Lu
Rev. B
17
Flowcell Tubing Replacement
The flowcell utilizes 1.2 mm I.D. Teflon tubing for the sample and exit tubes. Replacement
tubing is included with the tubing kit. Follow this procedure to replace the flowcell tubing.
1.
2.
3.
6.
iv
Flowc
Remove the flowcell. Unscrew the Luer and lift the flowcell out of the read well.
Remove the cover screws and lift off the upper flowcell cover.
Refer to Figure 5-A. Disconnect the coupling tube from the steel tube, then remove the
coupling tube from the exit tube. Pull the exit tubes out through the bulkhead. Remove
the cell insert screws and pull the cell insert and the sample tube out. Remove the
sample tube from the steel tube.
Select the long or the short sample tube. The short sample tube must be used when the
sample volume is set to 350 1] or less. Carefully press fit the end of the sample tube
with the red dot (swaged end) to the steel tube on the cell insert, and feed the other end
upward through the cell body. Hint: grasp the tubing with a small piece of #400 grit
emery paper. Do not kink the tubing. Refer to Figure 5-C for the proper orientation. Do
not reverse the orientation as improper sampling will result. Install the cell body and
screws.
Feed the exit tube in through the rear of the flowcell.Press a new coupling tube over
the end of the exit tube. The exit tube must be inserted at least 7 mm to ensure a leak-
free seal. Minimize the gap between the ends of the tubes as shown in Figure 5-B.
Press the coupling tube (exit tube) over the corresponding steel tube.
sell Adjustment
The flowcell must be adjusted after replacing the flowcell tubing, or any time the cell insert is
removed or the adjustment set screw is disturbed.
1.
2.
With the instrument on, lift the flowcell out of the read well. Do not unscrew the fitting.
Press MENU. Type 189 and press ENTER. The instrument will continuously report
the detector voltage at 405 nm. Record this value for reference in the next step.
Sample deionized water. Visually confirm there are no air bubbles in the cell window.
Insert the flowcell into the read well until it bottoms out. Note the value displayed. If
the displayed value is more than 50% greater than the value you recorded in step 2, no
adjustment is needed.
If the displayed value is less than 50% greater than the value in step 2, Removethe
flowcell. Adjust the set screw with the hex wrench supplied. Turn the set screw 1/4 turn
in either direction and go to step 4. If the value in step 4 increases, turn the set.screw in
the same direction. If it decreases, turn the setscrew in the opposite direction.
Repeat steps 4 and 5 until the displayed value is at a maximum.
When
complete,
press
CLEAR
twice
to
return
to
the
main
prompt.
You
must
read
new
water
values
as
described
in
"Flowcell
Configuration".
HE 2000 Service Manual