Human Humalyzer 2000 - Absorbance Calibration

36
Rev. B
Absorbance Calibration
Materials and equipment:
10.
12 mm borosilicate tube
Reference spectrophotometer
Reference material which peaks at one of the instrument's wavelengths.
Precise 1:2 serial dilution of the reference material.
Ensure that the flowcell is active. Press CELL. Press YES to activate the flowcell.
Press CLEAR twice to revert to the temperature display.
Place a 12 mm borosilicate test tube filied with 1 ml of water into the read well.
Select test #186 and confirm that all of the filter voltages are between 2.00 and 10.00
volts.
Determine the absorbance of both the 1:1 reference material and the 1:2 dilution on a
reference spectrophotometer. The 1:2 dilution should be in the range of .8 to 1.2 absor-
bance. The 1:1 material should be in the range of 1.6 to 2.4 absorbance.
Press ABS to place the instrument into the absorbance mode. Select the required wave-
lengths. Blank on the same diluent material used to prepare the concentrations.
Read the 1:2 dilution. Divide the absorbance as read on the reference spectrophotom-
eter by the reported absorbance value.
Exit the absorbance mode and select test #212. At the prompt “Absorbance factor=
x.xxx?”, enter the ratio calculated. When this factor is entered the instrument will
display a net factor (the new calibration data).
Press CLEAR twice and restart the absorbance mode. Blank as before on the diluent,
The 1:2 dilution absorbance should now agree with the reference to within +/-.005 -…"
absorbance.
Read the 1:1 concentration. It should agree within +/-2 % of the reference. If necessary,
carefully adjust trimpot TP3 to achieve this agreement. Re-blank and reconfirm the
values.
© Select test 4213 to print the new calibration data. Enter these values on the calibration
data label on the bottom of the instrument.
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: Humalyzer 2000 Service Munual