Integra DOSE IT - Chapter 4 Operation; Selection and Loading of the Tubing

DOSE IT – Operating instructions V08 Operation
10 INTEGRA Biosciences AG
4 Operation
The DOSE IT operates in different modes: DISPENSE mode for distribution of a defined
volume into containers and PUMP mode for continuous pumping of liquids. In addition,
customized applications can be defined (software version 2.0 and higher).
4.1 Selection and loading of the tubing
4.1.1 Tubing selection
The pumphead of the DOSE IT is compatible with differently sized tubing allowing the user
to dispense a wide range of dose volumes. The main criteria for the choice of tubing are
the speed and precision requirements of a specific application. The larger the inner
diameter (ID) of the tubing, the faster but less precise is the dispensing.
As a reference for selecting the appropriate inner diameter of the tubing, typical volume
and speed values for different tubing sizes are listed in Table 1.
N
OTE
The indicated volume values relate to the specified reproducibility and not to
the actual limits of the system. The values have been determined
experimentally and may vary slightly depending on the experimental set up.
Compatibility with the pumphead and specified reproducibility can be guaranteed for
original INTEGRA Biosciences’ tubing only.
N
OTE
8 mm ID tubing is only recommended for the “PUMP” program mode (for
accuracy reasons, drips between the aliquots and draw backs in the tubing
may be a problem).
Table 1: Typical volume and speed values
Tubing inner diameter (ID) 1 mm 2 mm 3 mm 4 mm 6 mm
Volume (ml) at CV* < 1 % > 0.5 > 1 > 3 > 7 > 15
Speed range (ml / min) 0.6 – 52 2.1 – 203 4.8 – 475 8.4 – 837 16 – 1634
* Coefficient of variation.