DOSE IT – Operating instructions V08 Maintenance
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5 Maintenance
5.1 Cleaning and servicing
WARNING
Always switch off the DOSE IT and disconnect it from the mains when
carrying out maintenance work.
If the DOSE IT gets soiled, it can be cleaned with a cloth moistened with soapy water or
with a 70 % solution of Ethanol.
Regular servicing of the DOSE IT by a qualified partner of INTEGRA Biosciences is
recommended.
5.2 Decontamination
The aspiration/dispensing tubes, tube collars and silicone tubing contacting the liquid can
be autoclaved at 121°C, 1 bar overpressure for at least 20 minutes. Silicone may become
brittle after extensive autoclaving. Replace the tubings if they are damaged.
If the surface of the DOSE IT has been in contact with biohazardous material, it must be
decontaminated in accordance to good laboratory practice. Do not spray directly on the
instrument but use a lint-free cloth, lightly soaked with a disinfectant and wipe dry directly
after decontamination. Never use acetone or other solvents! Follow the instructions
provided by the disinfectant manufacturer.
5.3 Leak Test
It is recommended to perform a leak test about every 3 months or when dosing errors
occur. A leak test will reveal worn or defect tubing or pumphead.
Procedure:
1) Load a silicone tubing in the pumphead.
2) Position the dispensing end of the tubing below the pumphead height into a container.
3) Put the aspiration tube in liquid and press P
RIME to fill the tubing completely without
any bubbles.
4) Lift the aspirating end of the tubing as high as possible above the pumphead. The
system is tight, if the liquid level do not decrease and no liquid flow out of the lower
dispensing end.
The moving parts of the pumphead should be
checked from time to time for freedom of movement.
Occasionally lightly grease the lever (1) and the
rollers (see image) with Teflon lubrication oil.
The rotor shaft runs on sealed bearings which do not
require lubrication.
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