DOSE IT – Operating instructions V08 Operation
14 INTEGRA Biosciences AG
Customize mode
The ‘Customize’ mode allows to program a customized application consisting of up to 20
single steps:
Pause Defines the duration of the interval between
doses in the dispensing mode. If switching to
manual, the dispensing of a dose has to be
activated manually.
0.1– 99.9 s,
manual
1.0 s
Direction Defines the rotational direction of the rotor and
hence the flow direction. / C W m e a n s c l o c k -
wise, / CCW means counter-clockwise.
/ C W
/ C C W
/ C W
Dispense
direction
Defines the rotational direction for the step type
“D
ISPENSE”. For the step type “ASPIRATION” the
rotor rotates oppositely.
/ C W
/ C C W
/ C W
No. of
heads
Defines the number of pump heads. 1, 2 1
Step 1–20 Defines single steps of a customized applica-
tion.
1–20 -
Step Description Parameter
Dispense Defines the dosing of liquids analog to the program mode
“Dispense”. The rotational direction is defined by the
parameter “Dispense direction’.
Volume
Repetitions
Flow rate
Pause
Dispense
Ramp
Defines dispensing with accelerating or decelerating
speed, needed e.g. for density gradients. Just define the
start and end flow to ramp the dispensing speed up or
down. If the start flow is identical to the speed of the previ-
ous step, and the pause of the previous step is set to man-
ual, the liquid can be dispensed without pause.
Volume
Repetitions
Pause
Start flow
End flow
Aspirate Defines the aspiration of liquids analog to the program
mode “Dispense” with opposite rotational direction.
Volume
Repetitions
Flow rate
Pause
Aspirate
Ramp
Defines the aspiration of liquids with accelerating or decel-
erating speed. Just define the start and end flow to ramp
the aspirating speed up or down. If the speed of the follow-
ing step is identical to the end flow, and the pause is set to
manual, the DOSE IT continues without pause.
Volume
Repetitions
Pause
Start flow
End flow
Parameter Description Range Default
To Next Page
Loading...
