6
Getting Started, Continued
Cell Mode
Parameters
Screen,
continued
The Cell Mode Parameters are described below:
Sensitivity (refers to the contrast of the objects from the
background). Adjusting the sensitivity higher makes the
instrument more sensitive to objects; useful for cells that do not
stain well with trypan blue while adjusting the sensitivity
lower makes the instrument less sensitive and is useful if there
is a lot of background.
Minimum cell size is used to select the lowest particle size to
include in the count. The size is set in 1 micrometer
increments. The lower range of the Countess
™
instrument is
5 μm. However, you can select a minimum cell size as low as
2 μm; when a cell size outside of the usual range is selected, the
numbers indicating particle size are shown in red.
Maximum cell size is used to select the maximum particle size
to include in the count. The size is set in 1 micrometer
increments. The maximum size that can be set is 60 μm.
Circularity is used to determine the objects to include in the
measurement based on roundness. Increasing the value from
80% requires objects to be more round for inclusion in the
measurement. Decreasing the value from 80% allows objects to
be less round. Adjusting this may be useful if the cell type is
not particularly circular or perhaps oddly shaped.
After modifying any parameters:
Press Apply to make the changes or,
Press Default to restore default parameters or,
Press Save to create a protocol. Save the protocol name and
user name in the appropriate fields, and press Save again. The
protocol is now saved and appears highlighted in the protocol
menu. To use the highlighted protocol, press Apply.
Continued on next page
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