General Guidelines
Countess
™
Cell Counting
Chamber
Slides
The Countess
™
cell counting chamber slides supplied with the
counter or available separately from Invitrogen (page 25) are
specially desig
ned for use exclusively with the Countess
™
automated cell counter. Use of other slides result in inaccurate cell
counts and can damage the Countess
™
instrument.
R
E
C
O
M
M
E
N
D
A
T
I
O
N
To obtain the best results, follow these recommendations:
Wear gloves during sample handling.
Do not touch the optical surfaces of the Countess
™
cell
counting chamber slides. Hold the chamber slides by the
edges.
Use the Countess
™
automated cell counter at room
temperature only (10–35ºC).
For accurate viability count results, ensure the counting area is
covered with cell suspension and count cells within 3 minutes
of mixing the cells with trypan blue solution as trypan blue is
toxic to cells. For best data with biological samples, we
recommend counting at least two samples and taking an
average.
The Countess
™
automated cell counter is supplied pre-
calibrated. To recalibrate your instrument, see page 20.
The Countess
™
automated cell counter memory holds one set
of data. Save your data to the USB drive after each reading.
You may transfer the data to your PC, using the USB drive
immediately as described in Transferring Data to a PC (page
16).
After usi
ng Countess
™
cell counting chamber slides,
appropriately dispose slides as biohazardous waste. Do not
reuse the chamber slides.
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