22
Troubleshooting
Problem Cause Solution
Sample handling
Do not insert the Countess
™
cell
counting chamber slide upside-down
as this may introduce liquid into the
instrument that could damage it.
Do not reuse the Countess
™
cell
counting chamber slides, as leftover
dye from the previous reading may
affect the next reading.
Do not use any other counting
chambers such as a glass
hemocytometer with the Countess
™
counter as it results in inaccurate cell
count and may damage the
instrument.
Ensure that the sample covers the
entire counting area and the
Countess
™
cell counting chamber
slide is inserted completely into the
counter.
Low and high
readings
The Countess
™
automated cell counter is
designed to read samples from 1 × 10
4
cells/mL to 1 × 10
7
cells/mL, with the
highest accuracy between 1 × 10
5
cells/mL
and 4 × 10
6
cells/mL.
If your sample is not in this range, you
may need to dilute the sample or add
more cells and read the sample again.
Poor image
quality
While viewing cells in the Zoom mode, use
the focus knob to adjust the image to
ensure that live cells have bright centers,
and dead cells have dark/blue centers.
Cell clumping Ensure the cells are not clumped.
Inaccurate cell
count
--
To maintain instrument sensitivity, we
recommend that you calibrate the counter
each year as described on page 21.
Continued on next page
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